DNA fingerprinting of mammalian cell lines using nonradioactive arbitrarily primed PCR (AP-PCR).

probe. Retention of the probe on the dialysis bag using the electroelution protocol and inefficient binding of the probe to glass beads (as seen with ds DNA below 0.5 kb in size) contribute to probe losses. Since the ss probe does not need to be purified from the LMP agarose gel, loss of the probe associated with the two established protocols described here (particularly with the glass bead protocol) is not a problem. The high yield and ease of recovery of the ss probe not only minimizes the number of steps in the S1 nuclease analysis, but also leads to a reducedexposure to radioactivity, since less radiolabeled ss product needs to be synthesized initially. Lastly, the high resolving capacity of NuSieve LMP agarose facilitates the preparation of a broad size range of ss DNA probes that do not need further concentration.